davood asgari
Abstract
Abstract
Background: Damage in cartilage tissue is one of the global challenges and affects a large percentage of people. Trauma or age-related degenerative disease is the main cause of osteoarthritis. The aim of this study was to evaluate the effect of intra-articular injection for bone marrow mesenchymal ...
Read More
Abstract
Background: Damage in cartilage tissue is one of the global challenges and affects a large percentage of people. Trauma or age-related degenerative disease is the main cause of osteoarthritis. The aim of this study was to evaluate the effect of intra-articular injection for bone marrow mesenchymal stem cells and the resulting secretions in repairing the damaged cartilage of the rat knee joint.
Methods: 30 male rats that weights approximately 200 to 250 g were randomly divided into five groups of 6 rats, including healthy group (receiving physiological serum), osteoarthritis group (monosodium iodoacetate injection), mesenchymal group, mesenchymal group + receiving the same volume of cell secretions + culture medium and culture medium injection group. Four weeks later, the rats have been killed and the hip joint examined by both macroscopic and microscopic methods.
Results: The results showed that the injection of 2.5*105 cells per ml had a good effect on the repairing of cartilaginous lesions caused by the monosodium iodoacetate injection. Histological studies showed that fibrillation rate, the presence of small gaps and deep gaps (P <0.05) decreased in the mesenchymal treatment group. The level of proteoglycans in mesenchymal treatment group increased significantly (P <0.05). Also, in the treatment group with mesenchymal secretions and culture medium, changes were observed in repairing the damaged cartilage, which was not significantly different from the osteoarthritis group.
Conclusion: In this study, the restorative effect has benn confirmed for bone marrow mesenchymal stem cells and their secretions in improving histopathological lesions of rat articular cartilage.
